2020-04-13 16:54:522025-05-10 09:51:40
Biological materials
Mutant
MGNA-A821 (yrbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/821 NBRP B. subtilis, Japan]
BKE27810 ([[gene|yrbD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
BKK27810 ([[gene|yrbD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
GP3556 (Δ[[gene|yrbD]]::phleo), available in [SW|Jörg Stülke]'s lab
MGNA-A821 (yrbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/821 NBRP B. subtilis, Japan]
BKE27810 ([[gene|yrbD]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27810 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
BKK27810 ([[gene|yrbD]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
References
The protein
Structure
[PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) [pubmed|30659158]